Chromatography is the term used to describe a separation technique in which a mobile phase carrying a
mixture is caused to move in contact with a selectively absorbent stationary phase. The objectives of this
study were analysis of the secondary metabolite products and evaluation antimicrobial activity. Bioactives
are chemical compounds often referred to as secondary metabolites. Eleven bioactive compounds were
identified in the methanolic extract of Fusarium chlamydosporum. DL-Arabinose, D-Glucose , 6-O-?-
D-galactopyranosyl, ?-D-Glucopyranoside , O-?-D-glucopyranosyl, 5-Hydroxymethylfurfural, N-(4,6-
Dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylidenea, 1H-Purin-2-amine , 6-methoxy-N-methyl, 2-Methyl-
9-?-d-ribofuranosylhypoxanthine, 1-Hexadecanesulfonic acid , 3,5,-dichloro-2,6-dimeth, Methyl-6,7-
benzoisoquinoline, Undeca -2,4,6,8,10-pentaenal , 11-(2-furyl)-,oxime, and 2-Bromotetradecanoic acid.
Malva sylvestris was very highly active 6.85±0.25 mm. The results of anti-fungal and anti-bacterial activity
produced by Fusarium chlamydosporum showed that the volatile compounds were highly effective to
suppress the growth of Aspergillus terreus (6.09±0.21) and Staphylococcus aureus (5.99±0.19) mm.