The aims of this research were analysis of the bioactive chemical products and evaluation of antibacterial and
antifungal activity. Bioactives (chemical compounds often referred to as secondary metabolites) were analyzed
using gas chromatography-mass spectroscopy (GC-MS) techniques, then the in vitro antibacterial and antifungal
activity of the methanolic extract was evaluated. Proteus mirabilis bioactive compounds were identified in the
methanolic extract of Proteus mirabilis. GC-MS analysis of Proteus mirabilis revealed the existence of the 1-
Cyclopropyl-3,4-epoxyhex -5-en-1-yne, Benzene , (ethylsulfonyl), Pyrazolo[1.5-a]pyridine , 3-methyl-2-phenyl , 1-
benzylindole , L-Proline , N-(cyclohexanecarbonyl)-,propyl , 3-Amino-7-nitro-1,2,4-benzotriazine 1-oxide , (+)-
trans-3,4-Dimethyl-2 -phenyltetrahydro-1,4-thiazine , Figure: Isophthalic acid ,di(2-methoxyethyl) ester ,
Isophthalic acid ,di(2-methoxyethyl) ester , 4-Benzyloxy-N-methylamphetamine, 1-(2-Benzyloxy-4-methyl- cyclohex-
3-enyl)-1 -methyl-ethylamine , Ethyl 4-([(E)-(2-nitrophenyl) methylidene]amino) benzoate , Tolpropamine ,
Methcathinone , 4-Dehydroxy-N-(4,5-methylenedioxy -2-nitrobenzylidene)tyram. The results of anti-fungal activity
produced by Staphylococcus aureus showed that the volatile compounds were highly effective to suppress the
growth of Aspergillus terreus.